PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants

PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants

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Summary: We present here PhotoGal4, a phytochrome B-based optogenetic switch for fine-tuned spatiotemporal control of gene expression in Drosophila explants.This switch integrates the light-dependent interaction between phytochrome B and PIF6 from plants with regulatory elements from the yeast Gal4/UAS system.We found that PhotoGal4 efficiently activates and deactivates gene expression upon red- or far-red-light irradiation, respectively.In addition, this optogenetic tc m350 tool reacts to different illumination conditions, allowing for fine modulation of the light-dependent response.Importantly, by simply focusing a laser beam, PhotoGal4 induces intricate patterns of expression in a customized manner.

For instance, we successfully sketched personalized patterns of GFP fluorescence such as emoji-like shapes or letterform logos in Drosophila explants, which illustrates the exquisite precision and versatility of this tool.Hence, we anticipate that PhotoGal4 will expand the powerful wall-e bearbrick Drosophila toolbox and will provide a new avenue to investigate intricate and complex problems in biomedical research.